ВИКОРИСТАННЯ ПРЕПАРАТУ “ОСТИВЕТ-II” ЗА ОСТЕОМІЄЛІТУ У СОБАК

Abstract

За результатами проведених досліджень доведено доцільність препарату «Остивет-II» для лікування гнійного остеомієліту у собак за різних варіантів його застосування. За місцевого післяопераційного промивання порожни¬ни гнійного остеомієліту у собак препаратом «Остивет-II» скорочуються терміни лікування завдяки ефективній антимікробній дії аквахелатів наночасток металів: Mg, Fe, Co, Cu, Zn, Ag; з їх вираженим анаболічним і стимулю- вальним мінералізацію ефектом. Застосування цих аквахелатів наночасток металів у складі желатинової пасти за пломбування гнійних остеомієлітних порожнин супроводжується найбільш вираженим стимулювальним ефектом, про що свідчать показники вмісту в крові загального білка, глюкози, загального кальцію, неорганічного фосфору.

По результатам проведенных исследований доказана целесообразность препарата «Остивет-II» для лечения гнойного остеомиелита у собак с различными вариантами его применения. При местном послеоперационном промывании полости гнойного остеомиелита у собак препаратом «Остивет-II» сокращаются сроки лечения благодаря эффективному антимикробному действию аквахелатов наночастиц металлов: Mg, Fe, Co, Cu, Zn, Ag; с их выраженным анаболическим и стимулирующим минерализацию эффектом. Применение этих аквахелатов наночастиц металлов в составе желатиновой пасты при пломбировании гнойных остеомиелитных полостей сопровождается наиболее выраженным стимулирующим эффектом, о чем свидетельствуют показатели содержания в крови общего белка, глюкозы, общего кальция, неорганического фосфора.

Researches of comparative efficiency of topical postoperative administration of an antibioticotherapia and nanoaq-uachelate-therapy were conducted by an admixture of metals: Mg, Fe, Co, Cu, Zn, Ag; at treatment of a posttraumatic purulent osteomyelitis at dogs. The fever, acceleration of respiration and pulse were observed in the first 5-10 days of a course of purulent osteomyelitis. Radiological accurately expressed sequestral cavity in which radiological taped the sequester was defined. At dogs of control group, since 9-10 days, and at dogs of experienced group, since 6-7 days, the body temperature, frequency of respiration and pulse were in limits of the upper bound of norm. At control dogs within 9-10 days, and at experienced within 5 - 6 days from fistulas observed allocation of a purulent exsudate with grains of "osteal sand" and fat droplets. Took place the expressed lameness of the leaning extremity. Established to palpation morbidity on all length of a bone, some tuberosity of its surface. By results of the carried-out treatment, the beginning of an lean on an extremity, in control group made 10,0 ± 0,48 days, in experienced group 7,1 ± 0,34 days (p <0,001). At the same time treatment duration in these groups made 19,4 ± 0,51 and 15,0 ± 0,67 days according to (p <0,001). The analysis of a peripheric blood of dogs of both groups showed that all studied indicators prior to experience statistically didn't differ from each other. Content of the general protein for the 5th days at dogs of control group on 2,54 g/l, and at dogs experienced on 5,01 g/l exceeded norm. For the 10th days these indicators were more respectively on 3,75 g/l and on 6,18 g/l, and for the 14th days the content of the general protein at dogs of the 1st group returned to norm, and at dogs 2nd groups on 5,33 g/l exceeded it. Thus, during the postoperative period at a purulent osteomyelitis there is an expressed intensification of production of proteins, and the admixture of nanoaquachelate of metals significantly strengthens this process. The glucose level augmentation in a blood from the moment of an operative measure and before the end of experience was observed in both groups of animals. However at dogs of experienced group contents in a glucose blood, in comparison with animals of control group, was more for the 5th days, on the 10th and for the 14th days. That is use of nanoparticles of metals for treatment of a posttraumatic purulent osteomyelitis is followed by distinct intensifying of power balance in an organism of dogs that is extremely important for providing a full-fledged current of reparative bone formation. Contents indicators during the preoperative period in both groups of animals were in a blood of the general calcium almost at the identical level within physiological norm. After carrying out a sequestrotomy, the level of the general calcium at dogs of control group underwent depression that, obviously, is caused by its costs of osteoreparative processes in connection with a gradual fusion of a sequestral cavity. Dogs of experienced group, after operation, in a blood of the general calcium, in comparison with control ani¬mals, had a contents more for the 5th days by 1,2 times, for the 10th days - by 1,1 times, for the 14th days - by 1,1 times. Content of inorganic phosphorus during the preoperative period was in a blood of dogs of both groups approximately at the identical level within norm. During the postoperative period this indicator experienced depression that is caused by mobi¬lization of phosphorus on processes of an osteoreparation. Use in treatment of a posttraumatic purulent osteomyelitis, after carrying out a sequestrotomy, an admixture of nanoaquachelate of metals, in comparison with an antibioticotherapy, was followed by reliable augmentation of contents in a blood of inorganic phosphorus for the 5th days by 1,2 times, for the 10th days by 1,4 times, on 14th days - by 1,1 times. The analysis of contents in a blood of the general calcium and inorganic phosphorus at a posttraumatic purulent osteo-myelitis at dogs of experienced group, in comparison with control dogs, demonstrates that the admixture of nanoaquachelate of metals enlarges level in a blood of both main the osteotropic of minerals - calcium and phosphorus which, in this regard are probably more intensively involved during osteoreparative process. Also researches of comparative efficiency of treatment of a purulent osteomyelitis, with formation of a sequestral box, by means of the condensed gelatinous sealing gel with addition of an above-mentioned admixture of nanoaquachelate of met¬als were conducted. Due to the chronic disease the body temperature, frequency of respiration and pulse were on the upper bound of norm. Observed allocations from fistulas of a small amount of a bloody and purulent exsudate with grains of "osteal sand" as a re-sult of disintegration of the sequester. Took place lameness of the leaning extremity. Among biochemical indicators in comparison with control, such shifts are found: 1) augmentation of content of the gen¬eral protein in the first experienced group for 12,5%, in the second experienced group for 8,8%; 2) augmentation of mainte¬nance of a glucose in the first experienced group for 17%, in the second experienced group for 15,5%; 3) augmentation of content of the general calcium in the first experienced group for 14,1%, in the second experienced group for 17,1%; 4) de¬crease of content of inorganic phosphorus respectively for 17,39% and 5,3%. Use of the condensed gelatinous sealing gel is followed by its gradual dissolution fabric enzymes during all medical pe-riod. Use of nanoaquachelate of metals as a part of gelatinous gel when sealing purulent the osteomyelitis cavities is followed by the most expressed stimulating effect what contents indicators in a blood of the general protein, a glucose, the general calcium, inorganic phosphorus testify to. The condensed (formalized) gelatin doesn't irritate a bone tissue, is slowly dissolved during the entire period of treatment, gradually giving the operating components. At the same time gelatin has certain medic¬inal properties as neutralizes (substrate - enzyme) proteolytic activity of originators of purulent inflammation. All this leads to noticeable acceleration of convalescence of sick animals which in the first experienced group made 43,4 ± 1,3 days, in the second experienced group 38,6 ± 0,49 days (in relation to the first experienced group р <0,01). At the same time in control groups the term of convalescence was 49,2 ± 0,58 and 48,4 ± 0,51 days respectively. The local postoperative lavage of a cavity of a purulent osteomyelitis at dogs the drug "Ostivet - II" reduces treatment terms thanks to effective antimicrobial action of aquachelate of nanoparticles of metals: Mg, Fe, Co, Cu, Zn, Ag; from them the anabolic and stimulating a mineralization effect expressed. Sealing of an osteomyelitis cavity formalized gelatin with addition of the drug "Ostivet - II" is an effective way in rational treatment of a purulent osteomyelitis at dogs.