PATHOGENICITY OF NON-PIGMENTED AND PIGMENTED ISOLATES OF P. AERUGINOSA DURING LONG-TERM STORAGE

Abstract

The aim: to study the pathogenicity of non-pigmented P. aeruginosa isolates compared to those that synthesize pigment during long-term storage for broiler chickens. Materials and methods. Bacteriological studies were carried out in accordance with generally accepted meth¬ods. Initially, P. aeruginosa and other bacterial microflora were isolated from poultry farms, pathological mate¬rial from poultry. The type of microorganisms was identified using Bergey's identifier. The obtained isolates were examined by morphological characteristics (according to Gram), tincture, cultural, biochemical, pathogen¬ic properties and sensitivity of selected cultures to antibiotics were studied. After the expiration of 3 years, the main biological properties of the isolates were monitored. To establish pathogenic properties, a bioassay was performed on white mice and one-week-old broiler chickens of the Cobb 500 cross (intraperitoneally by washout from daily agar culture at a dose of 0.2 cm3, which corresponds to the previously established LD50).