Effectiveness of aldehyde disinfectant against the causative agents of tuberculosis in domestic animals and birds

Abstract

In recent years conducted studies showed that mycobacteria are the most resistant microorganisms to negative environmental factors, physical and chemical effects. One of the major issues of animal tuberculosis is the correct selection of effective disinfectants. The aim of our studies was to estimate the interaction of aldehyde disinfectant on animal tuberculosis agents. It was found that the disinfectant, contained glutaraldehyde and formaldehyde, exhibits bactericidal properties on M. bovis and M. avium, as confirmed by culture, molecular genetics and electronic-microscopic research methods. The disinfectant partially destroyed the integrity surface of cellular structures in M. avium. The cytoplasm acquires density and contains vacuoles and finely granular substance. The area of nucleoid is blurred. In M. bovis cells the preparation causes destruction of cell surface structures on different poles, leading to the release of the cytoplasm and consolidation of the nucleoid area. Biocidal activity of the aldehyde disinfection preparation consists in the destruction of Mycobacterium surface structures, formation of vacuoles and osmiophilic granules in the cytoplasm. The aldehyde disinfectant impacts on mycobacterial cells with the comprehensive irreversible changes in structural elements that lead to the complete destruction of microorganisms.