Study of disinfectants bactericidal properties against Campylobacter spp. at sub-zero temperatures

Abstract

To provide an effective complex of veterinary and sanitary measures in farms, regardless of the livestock management and weather conditions, it is necessary to expand the range of disinfectants with expressed bactericidal properties. The research aimed to improve the disinfectant formulation and develop a method for determining its bactericidal properties at sub-zero temperatures. Based on the results, a method for determining the disinfectant activity at sub-zero temperatures was developed, which includes determining the bactericidal properties of the disinfectant against bacterial cells by suspension method, that includes several stages: preparing of bacterial suspension, preparing of test objects, contaminating of test objects and disinfecting at the temperature range from -20 to -1 °C. The developed disinfectant showed effectiveness at low temperatures and consists of active substances (glutaraldehyde, formaldehyde) at a concentration of 0.5% and antifreeze at a concentration of 20.0%. Bactericidal properties of new disinfectant have shown activity against Campylobacter fetus subsp. venerealis (Cfv) and Campylobacter fetus subsp. fetus (Cff) cultures at a concentration of 0.5% at an exposure of 30 minutes at temperatures -20°C. The obtained data supplemented the methodological approaches regarding testing new antimicrobial drugs and expanding the arsenal of effective disinfectants for veterinary medicine.